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At room temperature in TBS (25 mM Tris, pH 7.5, 150 mM NaCl containing 2 BSA) and then for another hour with TBS buffer containing 2 BSA, 0.1 Tween 20, and PIP2 antibody (1:2,500 dilution). The strips were washed 3 times for 5 min each with TBS containing 0.1 Tween-20. The strips were then incubated for 1 h with secondary antibody (IRDye 700) diluted 10,000 times in TBS with 2 BSA and 0.1 Twe

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